Related Experiment Video
Updated: Sep 20, 2026

Optimal Preparation of Formalin Fixed Samples for Peptide Based Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Workflows
Published on: January 16, 2018
[Application of high resolution mass spectra to the analysis of alpha-thiocarbonylthioformamides]
Jian-ping Zou1, Lu Wang, Chuan-qin Liu
1Department of Chemistry, Suzhou University, Suzhou 215006, China.
Abstract:
Acetophenone and its derivatives reacted on thionyl chloride to form an intermediate, which can react on the secondary aminnes to form alpha-carbonylthioformamides (1a-1f), followed by sulfurization with lawesson reagent to give target compounds alpha-thiocarbonylthioformamides (2a-2f). Compounds 2a-2f are the deep-red or deep-green crystals because there are two thiocarbonyl groups in the molecule, and they can be used as far-infraed adsorbent dyes, laser-Q switch and organic conductors. Moreover, they are very important intermediates, can react on reagents with amino or diamino groups to produce a variety of acyclic or heterocyclic compounds, which can be used as pesticides and medicines. Therefore, it is necessary to develop a new method for the analysis of alpha-thiocarbonylthioformamides (2a-2f). OA-TOF high resolution mass spectrometer is suitable to do this because of its small volume, high speed and giving elemental composition of ions. In this paper, the mass fragmentation pattern of alpha-thiocarbonylthioformamides was studied in detail using OA-TOF high resolution mass spectrometer of Micromass. The results showed that the molecular ions appeared, then, a neutral molecule S2 was lost easily from the molecular ion of alpha-thiocarbonylthioformamides, forming a particular ion M-S2 (base peak). This provides an effective method for the analysis of these compounds.
Related Concept Videos
High-Resolution Mass Spectrometry (HRMS)
Mass Spectrometry of Amines
Mass Spectrometry: Carboxylic Acid, Ester, and Amide Fragmentation
For example, the fragmentation of...
Mass Spectrum: Interpretation
NMR Spectroscopy and Mass Spectrometry of Aldehydes and Ketones
MALDI-TOF Mass Spectrometry

