Related Experiment Video
Updated: Aug 13, 2026
An In Vitro Enzymatic Assay to Measure Transcription Inhibition by Gallium(III) and H3 5,10,15-tris(pentafluorophenyl)corroles
Published on: March 18, 2015
[Synthesis characterization and DNA binding spectroscopy studies of cobalt(III) mixed-polypyridyl complex]
1Institute of Molecular Science, Shanxi University, Taiyuan 030006, China.
Abstract:
Two complexes of[Co(phen)2PIP](ClO4)3.H2O and [Co(phen)2HPIP](ClO4)3.2H2O have been synthesized(PIP = 2-imidazo[4,5-f][1,10]phenanthroline, HPIP = 2-(2-hydroxyphenyl)-imidazo [4,5-f][1,10]phenanthroline) and characterized by elemental analysis, UV, IR and 1H NMR spectroscopy. The interaction of the complexes with calf thymus DNA have been studied using absorption and emission spectroscopy, DNA melting techniques and cyclic voltammetric. The compounds show absorption hypochromicity, fluorescence enhancement, DNA melting increasement when binding to CT.DNA. CV measurement shows that the shift in reduction potential and the change in peak current with addition of DNA. These results prove the compounds insert DNA base pairs. The shift of peak potential indicates the ion interaction mode between the drug and DNA. The binding constants of the compounds to DNA are 1.8 x 10(4) and 4.37 x 10(4) respectively.
Related Concept Videos
02:51Metal-Ligand Bonds
In these complexes, transition metals form coordinate covalent bonds, a kind of Lewis acid-base interaction in which both of the electrons in the bond are contributed by a donor (Lewis base) to an electron acceptor (Lewis acid). The Lewis acid in...
02:34Structural Isomerism
Isomers are different chemical species that have the same chemical formula. Structural isomerism of coordination compounds can be divided into two subcategories, the linkage isomers and coordination-sphere isomers.
Linkage isomers occur when the coordination compound contains a ligand that can bind to the transition metal center through two different atoms. For example, the CN− ligand can bind through the carbon atom or through the nitrogen atom. Similarly, SCN− can be...
02:58Crystal Field Theory - Octahedral Complexes
To explain the observed behavior of transition metal complexes (such as colors), a model involving electrostatic interactions between the electrons from the ligands and the electrons in the unhybridized d orbitals of the central metal atom has been developed. This electrostatic model is crystal field theory (CFT). It helps to understand, interpret, and predict the colors, magnetic behavior, and some structures of coordination compounds of transition metals.
CFT focuses on...
03:02Colors and Magnetism
When atoms or molecules absorb light at the proper frequency, their electrons are excited to higher-energy orbitals. For many main group atoms and molecules, the absorbed photons are in the ultraviolet range of the electromagnetic spectrum, which cannot be detected by the human eye. For coordination compounds, the energy difference between the d orbitals often allows photons in the visible range to be absorbed and emitted, which is seen as colors by the human eye.
02:13Cooperative Binding of Transcription Regulators
00:43Complexometric Titration: Ligands