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[Induced expression of dopamine D2 receptor in human neuro-progenitor cell line HNG1210-E]
Qikuan Hu1, Changsheng Lin, Yun Bai
1Peking University Stem Cell Research Center, Beijing 100083, China.
Abstract
Objective:
To investigate whether the dopamine receptor D2 can express or can be induced to express in HNG1210-E, a monoclonal, telomerase-immortalized, human neural progenitor cell line.
Methods:
By means of RT-PCR, immuno-fluorescent staining, and fluo3 Ca2+ imaging to the expression of D2 mRNA and protein as well as the reaction to dopamine were demonstrated.
Results:
HNG1210-E cells could be induced to express D2 mRNA and its proteins. The induced cells also reacted to dopamine (5 mmol.L-1), which caused rapid rising of cytoplasm Ca2+.
Conclusion:
HNG1210-E cells can be induced to express D2 mRNA and functional proteins.