Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An expanded eukaryotic genetic code.

Jason W Chin1, T Ashton Cropp, J Christopher Anderson

  • 1Department of Chemistry and Skaggs Institute for Chemical Biology, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.

Science (New York, N.Y.)
|August 16, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

EVODEX: A Cheminformatics Framework for Mechanistic Abstraction and Prediction of Enzymatic Reactions.

Journal of chemical information and modeling·2026
Same author

Highly mutagenic continuous evolution in E. coli using a Φ29-based orthogonal replication system.

Nature biotechnology·2026
Same author

High-fidelity human chromosome transfer and elimination.

Science (New York, N.Y.)·2025
Same author

<i>Escherichia coli</i> with a 57-codon genetic code.

Science (New York, N.Y.)·2025
Same author

Genetic Code-Locking Confers Stable Virus Resistance to a Recoded Organism.

Biochemistry·2025
Same author

Automated orthogonal tRNA generation.

Nature chemical biology·2024
Same journal

Erratum for the Research Article "Detecting supramolecular organic nanoparticles during heat wave".

Science (New York, N.Y.)·2026
Same journal

Local signals, systemic decline.

Science (New York, N.Y.)·2026
Same journal

The mechanics of liver regeneration.

Science (New York, N.Y.)·2026
Same journal

Computing in a memory with physics.

Science (New York, N.Y.)·2026
Same journal

Retraction.

Science (New York, N.Y.)·2026
Same journal

Making time.

Science (New York, N.Y.)·2026
See all related articles

Scientists have developed a method to incorporate novel amino acids into yeast proteins using the TAG codon. This breakthrough enables new possibilities for protein engineering and studying cellular interactions.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Synthetic Biology

Background:

  • The genetic code's limitations restrict protein engineering.
  • Incorporating unnatural amino acids (UAAs) offers expanded functional capabilities.
  • Developing efficient methods for UAA incorporation in eukaryotes is crucial.

Purpose of the Study:

  • To establish a general and rapid method for UAA addition to the genetic code of Saccharomyces cerevisiae.
  • To demonstrate the efficient and high-fidelity incorporation of UAAs in response to the TAG nonsense codon.
  • To enable the creation of proteins with novel functionalities for various applications.

Main Methods:

  • Utilized the TAG nonsense codon for UAA insertion in Saccharomyces cerevisiae.
  • Engineered yeast strains to efficiently and accurately incorporate UAAs.

Related Experiment Videos

  • Synthesized UAAs with specific functional groups, including keto groups, heavy atoms, and photocrosslinkers.

    • Main Results:

    • Successfully incorporated five different UAAs into yeast proteins with high fidelity.
    • Demonstrated that the keto group on incorporated UAAs can be modified in vitro and in vivo.
    • Showcased the utility of UAAs for structural studies (heavy atom) and protein interaction analysis (photocrosslinkers).

    Conclusions:

    • This methodology overcomes genetic code constraints for protein manipulation in yeast.
    • Provides a foundation for expanding the genetic code in multicellular eukaryotes.
    • Opens new avenues for protein engineering and biological research.