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[Ziehl-Neelsen-Victoria blue-iodine green compound staining method showing leprotic bacillus]
Zhijin Gong1, Minghua Zhu, Jianming Zheng
1Department of Pathology, First Affiliated Hospital, Second Military Medical University, Shanghai 200433, China.
Objective:
To compare compound staining methods showing leprotic bacillus, elastic fibre and cell nucleus etc. in skin biopsy tissue.
Methods:
A compound staining dye composed of Ziehl-Neelsen Phenol Fuchsin, Victoria blue, iodine green and Martius yellow were used in paraffin-embedding skin biopsy tissue.
Results:
Leprotic bacilli showed red; cell nuclei, light green; cell cytoplasm, colorless; elastic fibres around tuberculoid nodules, blue; and essential substances, light yellow in skin biopsy tissue.
Conclusion:
Because the color was single in Ziehl-Neelsen staining method, its contrast was poor. The ZN-VB-IG compound staining method may show leprotic bacilli, elastic fibres and cell nuclei etc. distinctly. Therefore, this is a reliable and nice color contrast compound staining method.