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Updated: Aug 9, 2026
Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity
Published on: December 17, 2013
[Experimental study on cytotoxic activity of antigen specific cytokine induced killer cells]
Jiren Yu1, Yongjin Shi, Xinan Cen
1Department of Hematology, Peking University First Hospital, Beijing 100034, China.
Objective:
To prepare antigen specific cytokine induced killer(CIK) cells.
Methods:
Soluble antigen was prepared from tumor cells by repetitive freeze-thaw method. Cell killing activity is measured by MTT.
Results:
Cytotoxic activity was compared among CIK cells derived under three different conditions. (1) CIK cells prepared in the presence of the same tumor antigen fed dendritic cells (DC); (2) CIK cells prepared in the presence of DC without feeding the same tumor antigen; (3) CIK cells derived in the absence of DC. Among the three groups tested, DC-A-CIK cells showed the highest cytotoxic activity.
Conclusion:
Soluble target tumor antigen fed DC can significantly enhance the specificity and cytotoxicity of CIK cells to tumor target cells.
