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[Metallothioneins gene in induced mouse allergic contact dermatitis]
Xueyan Lu1, Linfeng Li, Wenhui Wang
1Department of Dermatology, Peking University Third Hospital, China.
Abstract
Objective:
To investigate the chronic hypersensitivity (CHS) to dinitrofluorobenzene (DNFB) in metallothionein gene knocked-out (MT -/-) mice and wild mice (MT +/+) with homologous genes.
Methods:
Mice were sensitized by applying DNFB on abdominal skin and CHS was induced on right ears. The CHS was observed by ear swelling.
Results:
In comparison with metallothionein--null mice, the ear swelling of normal control wild mice was significantly greater. (P < 0.05, Students t test).
Conclusion:
Metallothioneins play an important role in the CHS. Metallothionein gene knocked-out (MT -/-) mice express less inflammation of CHS.