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Related Experiment Videos

A Hox 3.3-lacZ transgene expressed in developing limbs.

B G Jegalian1, R W Miller, C V Wright

  • 1Department of Biological Chemistry, University of California, Los Angeles 90024.

Mechanisms of Development
|December 1, 1992
PubMed
Summary

Researchers created transgenic mouse lines using the Hox 3.3 Promoter II to track gene expression. These lines help distinguish cells in developing mouse embryos, aiding in understanding molecular differences.

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Area of Science:

  • Developmental Biology
  • Genetics
  • Molecular Biology

Background:

  • Understanding gene regulation is crucial for studying embryonic development.
  • Hox genes play vital roles in establishing body patterns during embryogenesis.
  • Identifying specific cell populations within the developing embryo is challenging.

Purpose of the Study:

  • To generate and characterize transgenic mouse lines expressing lacZ reporter gene under the Hox 3.3 Promoter II.
  • To investigate the regulatory elements of the Hox 3.3 Promoter II that control gene expression boundaries.
  • To develop tools for molecularly distinguishing cell populations in the mouse embryo.

Main Methods:

  • Generation of transgenic mouse lines using lacZ as a reporter gene.
  • Cloning and manipulation of the Hox 3.3 Promoter II DNA sequences (3.6 kb and 0.6 kb fragments).

Related Experiment Videos

  • Analysis of lacZ expression patterns in various embryonic tissues via X-gal staining.

    • Main Results:

    • A 3.6 kb promoter fragment correctly defined the anterior expression boundary in ectodermal and mesodermal tissues.
    • Posterior expression boundaries were not consistently respected, with expression extending into the tail.
    • Shorter promoter fragments (0.6 kb) resulted in ectopic expression in the head, including the retina.
    • One line exhibited strong graded expression in the anterior proximal limb bud.

    Conclusions:

    • The Hox 3.3 Promoter II contains regulatory elements that can define anterior expression boundaries in mouse embryos.
    • Variations in promoter fragment length influence expression patterns, leading to both expected and ectopic expression.
    • These transgenic mouse lines serve as valuable tools for molecularly identifying and studying cell populations during mouse embryogenesis.