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Lysosomal Hydrolases

Lysosomal Hydrolases

Lysosomes are the site for the degradation of macromolecules and biological polymers released during membrane trafficking events such as secretory, endocytic, autophagic, and phagocytic pathways. The membrane-enclosed area of the lysosome, called the lumen, contains hydrolytic enzymes active in an acidic environment. These acid hydrolases are functional at a pH between 4.5 and 5 and are involved in cellular processes such as cell signaling, energy metabolism, restoration of the plasma membrane,...

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Related Experiment Video

Updated: Nov 23, 2025

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Published on: June 23, 2022

Protocol for Probing Regulated Lysosomal Activity and Function in Living Cells.

L V Albrecht¹, N Tejeda-Muñoz¹, E M De Robertis¹

¹Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles 90095-1662, USA.

|December 30, 2020

Summary

This summary is machine-generated.

New imaging protocols quantify real-time lysosome function, including enzyme activity and pH. These methods overcome limitations of current lysosomal tracers for studying cellular catabolism.

Failed At:

2026-07-10T14:57:25.036073+00:00

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