Related Experiment Video
Updated: Aug 24, 2026
Application of the Intelligent High-Throughput Antimicrobial Sensitivity Testing/Phage Screening System and Lar Index of Antimicrobial Resistance
Published on: July 21, 2023
High-throughput protocol for assessing antibiotic resistance using agar dilution, acoustic liquid handling, and
Lieze Agten1, Lieselotte Vermeersch2, Philip Ruelens1
1KU Leuven, Department of Microbial and Molecular Systems (M2S), Centre of Microbial and Plant Genetics (CMPG), 3000 Leuven, Belgium; Symbiotic and Pathogenic Interaction Group, VIB Center for Microbiology, VIB, 3001 Leuven, Belgium.
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Conventional minimum inhibitory concentration (MIC) assays used to determine the antibiotic susceptibility of microorganisms are inherently low-throughput and labor intensive. Here, we present a high-throughput agar dilution MIC assay to efficiently assess resistance profiles across large collections of bacterial isolates. We describe steps for agar plate preparation, culture spotting, imaging setup, and semi-automated image-based data analysis of this high-throughput assay. This approach enables simultaneous determination of antibiotic resistance levels for 384 strains across multiple antibiotics within a single workflow. For complete details on the use and execution of this protocol, please refer to Van den Bergh et al.1.
