The influence of an internal electric field upon protein crystallization using the gel-acupuncture method
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Updated: Sep 20, 2026
Growing Protein Crystals with Distinct Dimensions Using Automated Crystallization Coupled with In Situ Dynamic Light Scattering
Published on: August 14, 2018
N Mirkin1, B A Frontana-Uribe, A Rodríguez-Romero
1Instituto de Química, UNAM Circuito Exterior, C. U. Mexico, D. F. 04510, Mexico.
Abstract:
In this work, the influence of an internal electric field upon the crystallization of lysozyme and thaumatin is explored using a modified design of the gel-acupuncture setup. From a crystallographic point of view, the orientation of crystals that grow preferentially over different types of electrodes inside capillary tubes is also evaluated. Finally, the crystal quality and the three-dimensional structure of these proteins grown with and without the electric field influence are analyzed by means of X-ray diffraction methods.
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