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Related Concept Videos

iChip01:24

iChip

The cultivation of environmental microorganisms has long been hindered by the inability to replicate complex native conditions in vitro. The isolation chip (iChip) addresses this limitation by facilitating the growth of previously uncultivable microorganisms through in situ incubation. Designed for high-throughput microbial cultivation, the iChip comprises hundreds of microchambers, each capable of housing a single microbial cell. These microchambers are loaded with a mixture of molten agar and...

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Related Experiment Video

Updated: Jul 28, 2026

In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells
05:57

In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells

Published on: May 16, 2011

Microbial ingrowth around single- and multi-component adhesives studied in vitro.

S Preussker1, W Klimm, M Pöschmann

  • 1Department of Conservative Dentistry, University Clinic Carl Gustav Carus, Fetscherstrasse 74, Dresden, Germany. susann.preussker@mailbox.tu-dresden.de

Caries Research
|August 20, 2003
PubMed
Summary

This study found low microbial leakage with all tested dental adhesive systems. Single-component adhesives performed comparably to multi-component ones in vitro.

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Evaluation of the Curing of Adhesive Systems by Rheological and Thermal Testing
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Published on: July 3, 2020

Area of Science:

  • Restorative Dentistry
  • Dental Materials Science
  • Microbiology

Background:

  • Dental composite restorations are prone to microleakage, potentially leading to secondary caries.
  • Evaluating the performance of single-component versus multi-component dental adhesives is crucial for clinical success.
  • Understanding microbial leakage patterns is essential for improving restoration longevity.

Purpose of the Study:

  • To compare in vitro microbial leakage of four micro-hybrid composites with four single-component and four multi-component dental adhesives.
  • To assess the marginal adaptation and leakage of different composite-adhesive systems.
  • To evaluate the influence of adhesive type and layering technique on microbial ingress.

Main Methods:

  • Ninety-four human premolar Class V cavities were restored using eight different composite-adhesive systems (one-layer or two-layer technique).
  • Restorations underwent thermocycling and incubation with Streptococcus mutans and Lactobacillus acidophilus.
  • Microbial leakage was quantified histologically after decalcification and staining.

Main Results:

  • Overall microbial leakage was very low across all tested groups, with no significant differences between adhesive types.
  • Z100 MP composite showed significantly higher leakage than Tetric Flow systems (p=0.037).
  • XRV Herculite systems exhibited significantly less leakage than Arabesk Top systems (p<0.001).
  • Single-component adhesives demonstrated marginal adaptation comparable to multi-component adhesives in vitro.

Conclusions:

  • Both single-component and multi-component dental adhesives provide comparable marginal adaptation in vitro.
  • Material selection (composite and adhesive) influences microbial leakage, with specific systems showing better performance.
  • Further clinical studies are warranted to validate these in vitro findings on microbial leakage.