Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Pan and sentinel lymph node visualization using a near-infrared fluorescent probe.

Lee Josephson1, Umar Mahmood, Patrick Wunderbaldinger

  • 1MGH/Harvard Medical School, Building 149, 13th Street, Charlestown, MA 02129, USA. ljosephson@partners.org

Molecular Imaging
|August 21, 2003
PubMed
Summary

A novel near-infrared fluorescent (NIRF) probe, Cy5.5-PGC, effectively visualizes lymph nodes for surgical guidance. This non-radioactive imaging method aids in identifying sentinel lymph nodes and reducing tissue damage during dissection.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Decoration of Autophagy Detecting Nanoparticle with an Anionic Fluorochrome Enhances Multispectral Characterization of Autophagosome Location and Flux.

Small (Weinheim an der Bergstrasse, Germany)·2024
Same author

Preclinical Evaluation of Novel Positron Emission Tomography (PET) Probes for Imaging Leucine-Rich Repeat Kinase 2 (LRRK2).

Journal of medicinal chemistry·2024
Same author

Radiochemistry for positron emission tomography.

Nature communications·2023
Same author

Imaging Leucine-Rich Repeat Kinase 2 In Vivo with <sup>18</sup>F-Labeled Positron Emission Tomography Ligand.

Journal of medicinal chemistry·2022
Same author

A nanoparticle probe for the imaging of autophagic flux in live mice via magnetic resonance and near-infrared fluorescence.

Nature biomedical engineering·2022
Same author

A novel monoacylglycerol lipase-targeted <sup>18</sup>F-labeled probe for positron emission tomography imaging of brown adipose tissue in the energy network.

Acta pharmacologica Sinica·2022

Area of Science:

  • Biomedical Imaging
  • Medical Diagnostics
  • Surgical Technology

Background:

  • Lymph node visualization is crucial for diagnosing metastases and minimizing tissue damage.
  • Current methods for lymph node imaging have limitations.
  • Sentinel lymph node biopsy is a key diagnostic procedure.

Purpose of the Study:

  • To evaluate a novel macromolecular near-infrared fluorescent (NIRF) probe, Cy5.5-PGC, for lymph node visualization.
  • To assess the probe's efficacy for both pan-node and sentinel node visualization.
  • To determine the probe's utility in guiding interventional radiologic and surgical procedures.

Main Methods:

  • Cy5.5-PGC was administered intravenously (i.v.) or subcutaneously (s.c.) into nude mice.
  • Near-infrared fluorescent (NIRF) and white light imaging were performed on live animals, partially dissected animals, and tissue specimens.

Related Experiment Videos

  • Image acquisition occurred 24 hours post-injection.

    • Main Results:

    • Intravenous Cy5.5-PGC allowed visualization of axillary lymph nodes in live mice.
    • Partial dissection revealed concentrated NIRF signals in iliac and aortic nodes.
    • Subcutaneous injection enabled clear visualization of draining axillary and brachial nodes.
    • NIRF imaging successfully visualized lymph nodes through tissue layers.

    Conclusions:

    • Cy5.5-PGC is an effective NIRF probe for visualizing lymph nodes via i.v. or s.c. administration.
    • NIRF imaging offers a non-radioactive alternative for lymph node detection and surgical guidance.
    • This technique has potential to improve accuracy and reduce invasiveness in nodal staging procedures.