Characterization of a small cryptic plasmid, pHP51, from a Korean isolate of strain 51 of Helicobacter pylori
Jae-Young Song1, Sang-Haeng Choi, Eun-Young Byun
1Department of Microbiology, Gyeongsang Institute of Health Science, Gyeongsang National University College of Medicine, 90 Chiram-dong, Jinju, Gyeongsangnam-do 660-751, South Korea.
Abstract:
The nucleotide sequence of a 3955-bp Helicobacter pylori plasmid, pHP51 was determined, and two open reading frames, ORF1 and ORF2, were identified. The deduced amino acid sequence of ORF1 was highly conserved (87-89%) among plasmid replication initiation proteins, RepBs. The function of ORF2 was not assigned because it lacked known functional domains or sequence similarity with other known proteins, although it had a HPFXXGNG motif that was also found in the cAMP-induced filamentation (fic) gene. Three kinds of repeats were present on the plasmid outside of the ORFs, including the R1 and R2 repeats that are common in H. pylori plasmids. One 100-bp sequence detected in the noncoding region of pHP51 was highly similar to the genomic sequence of H. pylori 26695.
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