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Related Experiment Videos

Skin facilitates Candida albicans mating.

Salil A Lachke1, Shawn R Lockhart, Karla J Daniels

  • 1Department of Biological Sciences, The University of Iowa, Iowa City, Iowa 52242, USA.

Infection and Immunity
|August 23, 2003
PubMed
Summary
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Candida albicans mating occurs on mouse skin, with up to 40% of cells fusing. Skin surface properties, not just temperature, facilitate this fungal process.

Area of Science:

  • Mycology
  • Microbial Pathogenesis
  • Cellular Biology

Background:

  • Candida albicans mating requires a switch to the opaque phase phenotype.
  • Opaque cells revert to the white phase at physiological temperature (37°C).
  • Opaque cells efficiently colonize skin, which is at a lower temperature than 37°C.

Purpose of the Study:

  • To investigate if mouse skin facilitates Candida albicans mating.
  • To explore the role of skin surface properties in fungal cell fusion.

Main Methods:

  • Incubation of a/a and alpha/alpha Candida albicans strains on newborn mouse skin under a cotton patch for 24 hours.
  • Utilizing scanning electron microscopy to visualize cell morphology and mating stages on the skin surface.
  • Control experiments on abiotic surfaces (glass, plastic, silicone) at 32°C.

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Main Results:

  • Significant Candida albicans mating was observed on mouse skin, with up to 40% of cells fused in some areas.
  • All mating stages observed in vitro were also present in vivo.
  • Unique morphological characteristics of mating on skin were noted, attributed to cell immobilization.
  • Fusion occurred at a very low frequency on abiotic surfaces, indicating skin-specific facilitation.

Conclusions:

  • Mouse skin provides a conducive environment for Candida albicans mating, independent of physiological temperature.
  • Unique features of the skin surface, beyond temperature, significantly promote fungal cell fusion.
  • This finding has implications for understanding fungal reproduction and colonization in vivo.