Evaluation of prostate-specific antigen (PSA) membrane test for forensic examination of semen

Prostate-specific antigen (PSA) is a glycoprotein produced by the prostatic gland and functions to liquefy the seminal fluid. Recently several PSA membrane test kits utilizing an immunochromatographic assay are commercially available for clinical screening of PSA concentration in serum. In this study, we applied the three PSA test kits to forensic examination of semen, and evaluated the sensitivity and specificity of the PSA test kits. The specificity were the same, but SMITEST PSA Card had the highest sensitivity among the three kits. Furthermore we certified that the SMITEST PSA Card was little affected by heat or contaminants such as other body fluids, reagent of preliminary test for semen stain and spermicides, and the PSA kit was applied to 30 casework samples.