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Related Concept Videos

Affinity Chromatography01:03

Affinity Chromatography

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Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
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Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
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Related Experiment Videos

Mixed-matrix membrane adsorbers for protein separation.

Maria-Elena Avramescu1, Zandrie Borneman, Matthias Wessling

  • 1Membrane Technology Group, Faculty of Chemical Technology, University of Twente, P.O. Box 217, NL 7500 AE Enschede, The Netherlands.

Journal of Chromatography. A
|August 27, 2003
PubMed
Summary

New ion-exchange mixed-matrix adsorber membranes effectively separate bovine serum albumin (BSA) and bovine hemoglobin (Hb). These membranes show high protein adsorption and efficient separation under various conditions.

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Area of Science:

  • Biochemistry
  • Materials Science
  • Chemical Engineering

Background:

  • Separating similar proteins like bovine serum albumin (BSA) and bovine hemoglobin (Hb) is challenging.
  • Developing advanced materials for efficient protein separation is crucial in biochemical applications.

Purpose of the Study:

  • To develop and evaluate novel ion-exchange mixed-matrix adsorber membranes for protein separation.
  • To investigate the impact of operational parameters on the separation performance of these membranes.

Main Methods:

  • Fabrication of mixed-matrix adsorber membranes by incorporating Lewatit ion-exchange resins into an ethylene-vinyl alcohol copolymer.
  • Testing separation of BSA and Hb using cation- and anion-exchange membranes.
  • Evaluating the effect of filtration flow-rate, pH, and ionic strength on separation efficiency.

Main Results:

  • The developed heterogeneous matrices exhibited high static and dynamic protein adsorption capacities.
  • High average separation factors for BSA-Hb were achieved at physiological ionic strength.
  • Effective separation was maintained up to a filtration flow-rate of 20 L/h/m² before protein breakthrough.

Conclusions:

  • Ion-exchange mixed-matrix adsorber membranes are a promising technology for separating similar proteins.
  • The membrane performance is significantly influenced by operational parameters like flow-rate and ionic strength.
  • These membranes offer efficient protein separation capabilities relevant for biochemical processes.