[Studies on the determination of plasma level and pharmacokinetic parameters of anisodamine by micellar liquid...

Anisodamine is a tropane alkaloid isolated from the plant Anisodus tanguticus (Maxim) Pasch. It is an anticholine drug widely used in clinics. Micellar liquid chromatography is a new type of HPLC developed in the 1980's. Direct plasma injection technique is the application of micellar HPLC in bioanalyses. In this paper, a micellar HPLC method, which employs n-propanol as modifier, SDS as surfactant, atropine sulphate as internal standard, has been developed. By direct injection, this method was successfully applied to the measurement of plasma level of anisodamine. Application of this method to the study of anisodamine pharmacokinetics was investigated in human volunteers following a single intramuscular injection. The separation was performed in a Shim-pack CLC-CN column (150 mm x 6 mm ID, 5 microns) with a mobile phase of n-propanol-water (15:85) with 45 mmol/L SDS and total ion strength 70 mmol/L by adding phosphate, and detected at 205 nm. The standard curve was linear over the concentration range of 15-750 ng plasma level (r = 0.9972). The measurable lowest concentration was 10 ng/ml plasma (S/N = 3:1). The study of anisodamine pharmacokinetic in man was also described.