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Related Experiment Videos

Multiplexed DNA quantification by spectroscopic shift of two microsphere cavities.

Frank Vollmer1, Stephen Arnold, Dieter Braun

  • 1Center for Studies in Physics and Biology, Rockefeller University, New York, New York 10021, USA. vollmef@rockefeller.edu

Biophysical Journal
|August 29, 2003
PubMed
Summary

We developed a novel biosensor using optical resonance in silica spheres for highly sensitive, label-free DNA quantification. This whispering gallery mode technique enables multiplexed detection and single nucleotide mismatch discrimination for advanced diagnostics.

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Area of Science:

  • Nanotechnology
  • Spectroscopy
  • Biotechnology

Background:

  • Label-free DNA quantification is crucial for molecular diagnostics.
  • Existing optical biosensors often lack the required sensitivity or multiplexing capabilities.
  • Microsphere-based resonators offer potential for enhanced biosensing.

Purpose of the Study:

  • To develop a novel, high-sensitivity, label-free DNA quantification technique.
  • To demonstrate the utility of whispering gallery mode (WGM) resonance in silica microspheres for nucleic acid detection.
  • To enable multiplexed DNA detection and single nucleotide mismatch discrimination.

Main Methods:

  • Chemically modifying silica microsphere surfaces with oligonucleotides.
  • Exciting and detecting optical resonances (WGM) in the microspheres.

Related Experiment Videos

  • Measuring wavelength shifts upon target DNA hybridization.
  • Utilizing unique resonance wavelengths for multiplexed detection.

    • Main Results:

    • Achieved a mass sensitivity of 6 pg/mm(2), exceeding many optical single-pass devices.
    • Demonstrated specific, multiplexed DNA detection using two distinct microspheres.
    • Successfully discriminated a single nucleotide mismatch in an 11-mer oligonucleotide with a high signal-to-noise ratio (54).

    Conclusions:

    • The developed all-photonic WGM biosensor provides a sensitive and specific method for DNA quantification.
    • The technique allows for multiplexed detection and high-resolution mismatch discrimination.
    • Integration onto a semiconductor chip promises a portable, manufacturable lab-on-a-chip device for diagnostics.