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Related Experiment Videos

Activity-evoked capacitative Ca2+ entry: implications in synaptic plasticity.

Atsushi Baba1, Takuya Yasui, Shigeyoshi Fujisawa

  • 1Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, Japan.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|August 29, 2003
PubMed
Summary

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Store-operated calcium entry (SOC) is present in hippocampal neurons, influencing NMDA receptor activity and synaptic plasticity. This finding reveals a new role for SOC in the central nervous system.

Area of Science:

  • Neuroscience
  • Cellular Biology
  • Calcium Signaling

Background:

  • Store-operated calcium entry (SOC) is a critical mechanism for calcium homeostasis in many cell types.
  • Its presence and function in central nervous system (CNS) neurons, particularly hippocampal pyramidal neurons, remain debated.
  • Understanding SOC in neurons is crucial for comprehending neuronal excitability and plasticity.

Purpose of the Study:

  • To investigate the presence and functional role of store-operated calcium entry (SOC) in cultured hippocampal pyramidal neurons.
  • To determine the impact of SOC on NMDA receptor-mediated calcium transients and synaptic plasticity in the hippocampus.

Main Methods:

  • Calcium imaging in cultured hippocampal pyramidal neurons.
  • Pharmacological inhibition of SOC channels using 2-aminoethoxydiphenyl borate (2-APB), SKF96365, and La3+.

Related Experiment Videos

  • Electrophysiological recordings and calcium imaging in hippocampal slice preparations to assess synaptic plasticity.

    • Main Results:

    • Evidence for SOC presence in hippocampal pyramidal neurons was demonstrated through calcium imaging.
    • SOC channel inhibitors prevented thapsigargin-induced calcium influx and accelerated the decay of NMDA-induced calcium transients.
    • Inhibition of SOC attenuated dendritic calcium accumulation and long-term potentiation at Schaffer collateral-CA1 synapses.

    Conclusions:

    • Hippocampal neurons possess functional store-operated calcium entry (SOC) channels.
    • SOC is linked to NMDA receptor activity and plays a significant role in modulating neuroplasticity.
    • These findings establish a novel connection between SOC and synaptic plasticity in the CNS.