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Purification of erythrocyte cytochrome P-450 from goat and chick
S S Patil1, S J Makhija, S S Pawar
1Chemistry Department, S.B.E.S. Science College, Aurangabad.
Indian Journal of Biochemistry & Biophysics
|December 1, 1992
Abstract:
Cytochrome P-450 has been purified from goat and chick erythrocytes and characterized. Goat erythrocyte cytochrome P-450 content was higher than that of chick erythrocytes cytochrome P-450. Elution profile of purified protein from DEAE-cellulose column showed a single peak. The catalytic activities of aminopyrine-N-demethylase and acetanilide hydroxylase were found to be higher in purified proteins. Molecular weight was determined by SDS-polyacrylamide gel electrophoresis.