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Updated: Jul 13, 2026

The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective
Published on: June 16, 2022
[Development of nutrient medium for the cultivation of Lactobacillus plantarum 8R-A3 ]
I B Sorokulova1, T V Khil'ko, A I Osadchaia
1Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, 154 Zabolotny St., Kyiv, 03143, Ukraine.
Abstract:
Optimal nutrient medium providing accumulation of cell biomass of Lactobacillus plantarum 8R-A3 has been developed. According to this index the proposed medium meets the nutrition requirements of bacteria under study and is comparable with the basal (MRS) medium. Dependence of growth properties of Lactobacillus plantarum 8R-A3 on concentration of the medium basal components has been studied. Maize extract (6 vol.%) and enzymolysate of fodder yeast (5 vol.%) are the most favourable sources of nitrogen nutrition. Addition of pantothenic, nicotinic acids or riboflavin to the nutrient medium increased the yield of Lactobacillus plantarum biomass.
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