Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Routine application of HLA class II oligotyping. Value of automatization].

S Caillat-Zucman1, J F Bach

  • 1INSERM U 25, Hôpital Necker, Paris.

Presse Medicale (Paris, France : 1983)
|December 2, 1992
PubMed
Summary

Automated HLA class II typing using DNA amplification and oligonucleotide hybridization improves accuracy and efficiency for large-scale analysis. This robotic system enhances sample preparation, reducing human error in genetic testing.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[T CD57+ lymphocytes in kidney transplanted patients. Phenotypic and functional study].

Presse medicale (Paris, France : 1983)·1992
Same author

Evidence that antihuman tumor necrosis factor monoclonal antibody prevents OKT3-induced acute syndrome.

Transplantation·1992
Same author

Age-dependent HLA genetic heterogeneity of type 1 insulin-dependent diabetes mellitus.

The Journal of clinical investigation·1992
Same author

A1B8DR3-associated CD8-positive T-cell expansion in HIV infection.

Lancet (London, England)·1992
Same author

[A new approach of prevention: predictive medicine].

Pathologie-biologie·1992
Same author

Combined effects of a thymic peptide, thymopoietin and myasthenic patient sera in rat myotube culture.

Journal of the neurological sciences·1992

Area of Science:

  • Immunogenetics
  • Molecular Biology
  • Biotechnology

Context:

  • Human Leukocyte Antigen (HLA) class II typing is crucial for transplantation and disease association studies.
  • Traditional methods can be labor-intensive and prone to human error.
  • Advancements in DNA amplification and hybridization offer improved typing accuracy.

Purpose:

  • To implement an automated system for HLA class II typing.
  • To enhance the efficiency and accuracy of large-scale genetic analyses.
  • To reduce the risk of errors in molecular typing procedures.

Summary:

  • Developed an automation system using the Biomek 1,000 robotic workstation for HLA class II typing.
  • Combined DNA amplification with allele-specific oligonucleotide hybridization for precise typing.

Related Experiment Videos

  • The automated system enables rapid preparation of numerous consecutive samples.

    • Impact:

    • Significantly improves overall typing accuracy in HLA class II analysis.
    • Facilitates large-scale genetic studies by increasing throughput and reliability.
    • The implemented automation system is now routinely utilized in multiple laboratories, demonstrating its practical value.