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Updated: Sep 20, 2026
Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
Published on: February 25, 2010
Concanavalin A-agarose removes mannan impurities from an extracellularly expressed Pichia pastoris recombinant
Małgorzata Palczewska1, Gyula Batta, Patrick Groves
1Nencki Institute of Experimental Biology, 3 Pasteur Street, 02-093 Warsaw, Poland.
Abstract:
Pichia pastoris secretes few native proteins. However, the more than 1 g l(-1)of extracellularly expressed mannan interfered with the purification of our extracellularly expressed, non-glycosylated recombinant protein. Concanavalin A-agarose removed more than 95% of the unwanted mannan as monitored by phenol reaction. A(13)C-based NMR assay confirmed this improvement. Concanavalin A-agarose can assist the purification of extracellular expressed, non-glycosylated proteins from yeasts.
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