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Published on: September 30, 2016
The application of FPLC chromatography (Mono Q) for the purification of wheat RNA polymerases II and III
Paweł Glanc1, Mariola Galbas, Piotr Dullin
1Department of Biochemistry and Biotechnology, Agricultural University, Wołyńska 35, 60-637 Poznań, Poland.
Cellular & Molecular Biology Letters
|September 2, 2003
Abstract:
Transcription is the main step in the regulation of gene expression. To study this process in vitro, it is necessary to obtain highly purified RNA polymerases. Here, we describe a method of RNA polymerase purification using a Mono Q FPLC column. Using Mono Q column chromatography accelerates the purification process and separates RNA polymerase II from RNA polymerase III with good yield.
