Examination on Candida spp. in refractory periapical granulomas

Area of Science:

• Oral pathology
• Microbiology
• Molecular biology

Background:

• Periapical granulomas are inflammatory lesions at the root apex.
• The presence of microorganisms in these lesions is a subject of ongoing research.
• Candida species are known opportunistic fungi with potential roles in various infections.

Purpose of the Study:

• To determine the occurrence of Candida species in refractory periapical granulomas.
• To utilize molecular and histological techniques for accurate detection.

Main Methods:

• Molecular analysis using polymerase chain reaction (PCR) with specific primers for Candida detection.
• DNA extraction from 103 surgically removed periapical granulomas.
• Histological and immunohistological examinations for confirmation.
• Use of beta-actin primers for DNA extraction control.

Main Results:

• Successful DNA extraction was achieved in 68 out of 103 samples.
• PCR indicated possible Candida occurrence in 18 samples.
• Subsequent PAS staining and immunohistochemistry were negative for Candida.
• Further analysis revealed PCR products were not typical for Candida species.

Conclusions:

• Candida species were not detected in the examined periapical granulomas.
• The findings suggest that Candida does not appear to be a common inhabitant of these lesions.