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Updated: Jun 9, 2026

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
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A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

Published on: August 25, 2017

Sequence-specific modification of genomic DNA by small DNA fragments

Dieter C Gruenert1, Emanuela Bruscia, Giuseppe Novelli

  • 1Department of Medicine, University of Vermont, Burlington, Vermont, USA. dieter@cooper.cpmc.org

The Journal of Clinical Investigation
|September 4, 2003
PubMed
Summary

Small DNA fragments enable precise genomic editing through small-fragment homologous replacement (SFHR). This gene editing technique shows promise for treating inherited diseases like cystic fibrosis and beta-thalassemia.

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