[Determination of Avermectin B1 content by first-order derivative spectrum analysis]

Area of Science:

• Analytical Chemistry
• Spectroscopy
• Pharmaceutical Analysis

Background:

• Accurate quantification of Avermectin B1 is crucial for pharmaceutical quality control.
• Existing methods may be susceptible to variations across different preparation forms.
• Development of a robust and reliable analytical method is needed.

Purpose of the Study:

• To develop and validate a novel quantitative method for Avermectin B1.
• To assess the method's reliability across different Avermectin B1 preparation forms.
• To establish a precise analytical approach for Avermectin B1 content determination.

Main Methods:

• Acquisition of ultraviolet absorption spectra and first-order derivative spectra of Avermectin B1 using a UV-2401 PC spectrophotometer.
• Identification of a stable spectral feature (peak at 252.2 nm and valley at 256.8 nm) unaffected by preparation form.
• Calculation of delta A (A252.2 - A256.8) as the basis for quantitative analysis and development of a standard regression equation.

Main Results:

• The spectral coordinates of the peak (A252.2) and valley (A256.8) remained consistent across different Avermectin B1 preparations.
• The derived quantitative analysis parameter, delta A, proved reliable for error reduction.
• A standard regression equation (c = 272.7 delta A - 0.161) with a high correlation coefficient (r = 0.9996) was established.

Conclusions:

• The developed spectrophotometric method utilizing delta A is highly reliable for Avermectin B1 quantification.
• This method demonstrates excellent suitability for determining Avermectin B1 content in diverse pharmaceutical preparations.
• The technique offers an accurate and robust approach for quality control of Avermectin B1.