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Functional Imaging of Viral Transcription Factories Using 3D Fluorescence Microscopy
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Published on: January 18, 2018

Transcriptional activation of the human immunodeficiency virus long terminal repeat sequences by tumor necrosis

V Zoumpourlis1, A G Eliopoulos, D A Spandidos

  • 1Institute of Biological Research and Biotechnology, National Hellenic Research Foundation, Athens, Greece.

Anticancer Research
|November 1, 1992
PubMed

The recombinant plasmid pBHIV-1 carrying the long terminal repeat (LTR) of the human immunodeficiency virus 1 (HIV-1), linked to the reported chloramphenicol acetyl transferase (CAT) gene, was introduced into human and rat fibroblasts. Stable transfectants were obtained which were resistant to genetecin and expressed CAT-activity from the HIV-1 LTR. The response to TNF alpha was studied. It was found that, at the optimum concentration of 100 IU/ml in human and 1000 IU/ml in rat fibroblasts, the expression of CAT was stimulated by 2.1 and 2.5-fold respectively. Our findings suggest that TNF-alpha in physiological concentrations can transcriptionally activate the HIV-1 LTR sequences and this may play an important role in the pathogenesis of HIV infection.

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