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CCL16 activates an angiogenic program in vascular endothelial cells.
Marina Strasly1, Gabriella Doronzo, Paola Cappello
1Division of Molecular Angiogenesis, Institute for Cancer Research and Treatment (IRCC), University of Turin, School of Medicine, Candiolo, Italy.
Blood
|September 6, 2003
Summary
Chemokine CCL16 activates angiogenesis by engaging CCR1 on vascular endothelial cells, promoting cell migration and capillary-like structure formation. This chemokine plays a role in liver vascularization and disease-related angiogenesis.
Area of Science:
- Cell Biology
- Immunology
- Vascular Biology
Background:
- Chemokines regulate leukocyte trafficking and can influence angiogenesis.
- The specific role of CCL16 in angiogenesis is not fully understood.
Purpose of the Study:
- To investigate the role of chemokine CCL16 in activating angiogenic programs in vascular endothelial cells.
- To identify the specific chemokine receptor involved in CCL16-mediated angiogenesis.
Main Methods:
- Endothelial cell migration and proliferation assays.
- In vitro capillary-like structure formation assay.
- Chick chorionallantoic membrane assay for in vivo angiogenesis.
- Use of CCR1 antagonist BX471 and blocking antibodies.
Main Results:
- CCL16 induces dose-dependent migration of endothelial cells without proliferation.
- CCL16 promotes endothelial differentiation into capillary-like structures and is angiogenic in vivo.
- CCL16 amplifies endothelial response to VEGF-A and increases production of CXCL8 and CCL2.
- CCR1 antagonism inhibits CCL16-induced angiogenesis, while CCR2 or CCR8 blockade does not.
Conclusions:
- CCL16 directly activates an angiogenic program in vascular endothelial cells via CCR1.
- CCL16 contributes to angiogenesis, particularly in the liver, and may be involved in hepatic vascular development and disease.