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Related Experiment Video

Updated: Mar 17, 2026

Human Adipose Tissue Micro-fragmentation for Cell Phenotyping and Secretome Characterization
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Human Adipose Tissue Micro-fragmentation for Cell Phenotyping and Secretome Characterization

Published on: October 20, 2019

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Secretome From Human Micro-Fragmented Adipose Tissue Affects In Vitro Monocytes/Macrophages Inflammatory Activity by

Valentina Coccè1, Eleonora Martegani2,3, Francesca Paino1

  • 1Department of Biomedical, Surgical and Dental Sciences - CRC StaMeTec, University of Milan, Milan, Italy, unimi.it.

Mediators of Inflammation
|March 16, 2026
PubMed
Summary
This summary is machine-generated.

The secretome from micro-fragmented adipose tissue (MFAT) shows anti-inflammatory effects by reducing inflammatory cytokines and cell adhesion molecules. This suggests MFAT secretome

Keywords:
ICAMMCP-1RANTESinflammationmicro-fragmented adipose tissuesecretome

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Area of Science:

  • Regenerative Medicine
  • Tissue Engineering
  • Cell Biology

Background:

  • Micro-fragmented adipose tissue (MFAT) is a promising biological preparation for regenerative medicine.
  • Its clinical effectiveness is linked to regenerative cells and growth factors.
  • The paracrine effects of MFAT secretome warrant investigation for therapeutic potential.

Purpose of the Study:

  • To investigate the biological activity of the secretome derived from cultured MFAT.
  • To assess its impact on inflammatory cytokine production (RANTES, MCP-1).
  • To evaluate its effect on macrophage cell adhesion molecule (CAM) expression and cell proliferation.

Main Methods:

  • ELISA assays were used to measure cytokine production.
  • Flow cytometry analyzed cell adhesion molecule expression on U-937 macrophages.
  • Cell proliferation assays assessed the impact on normal and cancer cells.

Main Results:

  • MFAT secretome inhibited MCP-1 and RANTES production.
  • It significantly reduced ICAM-1 expression on U-937 macrophages.
  • No impact was observed on the proliferation of normal or cancer cells.

Conclusions:

  • MFAT secretome exhibits anti-inflammatory properties.
  • It demonstrates a favorable safety profile regarding cell proliferation.
  • Paracrine effects of MFAT secretome may contribute to its efficacy in treating joint inflammation.