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Highly sensitive PCR assay for routine diagnosis of African swine fever virus in clinical samples
M Agüero1, J Fernández, L Romero
1Centro de Investigación en Sanidad Animal (CISA-INIA), Valdeolmos, 28130 Madrid, Spain. aguero@inia.es
Journal of Clinical Microbiology
|September 6, 2003
Abstract:
This work provides a novel, highly sensitive, hot start PCR method for rapid and specific detection of African swine fever virus (ASFV) that can be used as a routine diagnostic test for ASFV in surveillance, control, and eradication programs. A confirmatory test of the specificity of this method based on restriction endonuclease analysis was also developed.
