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Shell vial culture as a tool for isolation of Brucella melitensis in chronic hepatic abscess
Clarisse Rovery1, Jean Marc Rolain, Didier Raoult
1Service de Maladies Infectieuses et de Médecine Tropicale, Hôpital Nord, AHPM, Marseille, France.
Journal of Clinical Microbiology
|September 6, 2003
Abstract:
A 50-year-old man was admitted with a liver abscess and positive serology for Brucella spp. Liver pus and blood cultures remained sterile on conventional culture. Inoculation of liver pus onto eukaryotic cells by the centrifugation-shell vial technique yielded Brucella melitensis, identified by 16S rRNA gene amplification and sequencing.

