[Raman spectroscopic study of microscopic damage on the space structure of DNA with acetic acid]

The character of microscopic damage on the space structure of the herring sperm DNA by wetting treatment of acetic acid with Raman spectroscopic technique was reported. The Raman characteristic frequency and intensity were changed in different degree after wetting treatment on DNA by acetic acid. The results showed that the entire DNA molecule was damaged by acetic acid including backbone phosphodiester groups, deoxyribose and base stacking. The conformation and forms of DNA were changed, hydrogen bond was broken, B-form was modified, both single and double chains were disrupted. Base pairs were damaged. The damage degrees of the base pairs in turn were cytosine, thymine, guanine and adenine.