Related Experiment Video
Updated: Sep 20, 2026
Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
Published on: July 17, 2021
Y-chromosomal short tandem repeats haplotyping from vaginal swabs using a chelating resin-based DNA extraction method
Mineo Iwasa1, Hiroyoshi Koyama, Tsukasa Tsuchimochi
1Department of Forensic Medical Science, Nagoya City University Graduate School of Medical Sciences, Kawasumi 1, Mizuho-ku, Japan. nagaom@med.nagoya-cu.ac.jp
Abstract:
Reported are 2 autopsy cases in which Y-chromosomal microsatellite short tandem repeats DYS19, DYS389I and II, DYS390, and DYS393 could be haplotyped with vaginal swabs by using a Chelex 100-based DNA extraction method and dual-round polymerase chain reaction. The extraction of DNA from vaginal swabs by using this method was as efficient or more efficient than using proteinase K and phenol-chloroform extraction or the alkaline lysis methods. Y-chromosomal microsatellite short tandem repeats haplotyping based on the dual-round polymerase chain reaction method provided genotypes from all the loci determined. Although amplification of Y-chromosomal microsatellite short tandem repeats loci is not directly involved in the existence of spermatozoa, it is considerably advantageous for male individualization from body fluid mixture stains in criminal cases.
More Related Videos
08:22A Robust Polymerase Chain Reaction-based Assay for Quantifying Cytosine-guanine-guanine Trinucleotide Repeats in Fragile X Mental Retardation-1 Gene
Published on: September 16, 2019
09:39DNA Fingerprinting of Mycobacterium leprae Strains Using Variable Number Tandem Repeat (VNTR) - Fragment Length Analysis (FLA)
Published on: July 15, 2011
