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Updated: Aug 18, 2026

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination
Published on: May 13, 2017
[Fluorescence spectrometric determination of fleroxacin by charge-transfer reaction]
Li-ming Du1, Zhe-feng Fan, Rui-feng Zhang
1Center of Analysis and Test, Shanxi Normal University, Linfen 041004, China.
Abstract:
A simple and reliable fluorescence spectrometric method for the determination of fleroxacin (FLX) has been developed based on the charge-transfer reaction. The charge-transfer complex which formed between fleroxacin as the donor and 2, 3-dichloro-5, 6-dicyano-1, 4-benzoquinone (DDQ) as the acceptor has been studied by fluorimetry. Experiment shows that FLX is reacted with DDQ in a mixture of acetone and methanol at 30 degrees C for 30 min and a complex is formed. The composition of the charge-transfer complex is found to be 1:1 by Bent-French and curved intersection methods. The linear dynamic range is 0.4-8.4 mg.L-1, the detection limit is 0.1 mg.L-1 and the recovery of FLX is 96.6%-99.2%. At last, the charge-transfer reaction mechanism was explored. The method has been applied to the determination of FLX in tablets with satisfactory results.
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