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Comparison of di-n-methyl phthalate biodegradation by free and immobilized microbial cells
Jian Long Wang1, Yu-Cai Ye, Wei-Zhong Wu
1Laboratory of Environmental Technology, Institute of Nuclear Energy Technology, Tsinghua University, Beijing 100085, China. wangjl@tsinghua.edu.cn
Objective:
To compare the biodegradation of di-n-methyl pathalate by free and immobilized microbial cells.
Methods:
The enrichment and isolation technique was used to isolate the microorganism. The PAV-entrapment method was utilized to immobilize the microorganisms. The scanning electron microscophy (SEM) was used to observe the growth and distribution of microbial cells immobilized inside the PVA bead gels. The GC/MS method was used to identify the main intermediates of DMP degradation.
Results:
The microbial cells could grow quite well in PVA gel. The metabolic pathway did not change before and after immobilization of the microbial cells. The degradation rate of immobilized cells was higher than that of free cells.
Conclusion:
The immobilized microbial cells possess advantages than free cells when applied to the biodegradation of toxic organic pollutants.
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