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Published on: August 9, 2024
Hypertonic activation of a non-selective cation conductance in HeLa cells and its contribution to cell volume
Frank Wehner1, Takahiro Shimizu, Ravshan Sabirov
1Department of Cell Physiology, National Institute for Physiological Sciences, 444-8585 Okazaki, Japan. frank.wehner@mpi-dortmund.mpg.de
Abstract:
In whole-cell recordings on single HeLa cells, the hypertonic activation of a cation conductance with a selectivity ratio P(Na):P(Li):P(K):P(Cs):P(NMDG):P(Ca):P(Cl) of 1.00:0.86:0.84:0.56:0.10:0.07:0.15 was observed. This (non-selective) cation conductance was reduced to 59 and 30% of maximal stimulation by Gd(3+) and flufenamate, respectively, but it was insensitive to amiloride (with each compound applied at 100 microm/l). As was determined by the Coulter counter technique, the cation conductance was the main mechanism of regulatory volume increase (RVI) in HeLa cells. Whereas a significant contribution of Na(+)/H(+) antiport was also detectable, Na(+)-K(+)-2Cl(-) symport most likely did not contribute to RVI.
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