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[Effects of naloxone on rat cortical neuron apoptosis induced by anoxia]
Yang Song1, Hong Shen, Ai-shi Ding
1Department of Emergency, General Hospital of People's Liberation Army, Beijing 100853, China.
Objective:
To investigate the damage of anoxia on the cultured rat's cortical neurons and the protective effects of naloxone.
Methods:
Cortical neurons cultured for 12 days were randomly divided into three groups: control, anoxic group and anoxic group plus naloxone treated. Cortical neurons were exposed to anoxic environment for 6 hours and then cultured for 24 hours under normoxic condition. The apoptosis of neurons was detected with the method of TdT-mediated dUTP nick end labeling (TUNEL) and the apoptotic rate of neurons was measured using flow cytometry.
Results:
Anoxia could lead to increasing apoptosis and death of rate cortical neurons. Naloxone could reduce neuron apoptosis. There was a significant difference between the anoxic group and anoxic group plus naloxone (P<0.01).
Conclusion:
Naloxone can ameliorate the toxicity damage of neurons caused by anoxia through reducing neuron apoptosis, which suggests that naloxone could protect rat's cortical neurons from damage under anoxia condition.
