Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mouse ADAM33: two splice variants differ in protein maturation and localization.

Shelby P Umland1, Yuntao Wan, Himanshu Shah

  • 1Departments of Allergy and of Statistics, Schering-Plough Research Institute, Kenilworth, NJ 07033, USA. shelby.umland@spcorp.com

American Journal of Respiratory Cell and Molecular Biology
|September 16, 2003
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Population Pharmacokinetics of Clesrovimab in Preterm and Full-Term Infants.

Clinical pharmacology and therapeutics·2026
Same author

Imipenem/cilastatin/relebactam dosing regimen justification using exposure-efficacy analyses in participants with hospital-acquired bacterial pneumonia or ventilator-associated bacterial pneumonia in the RESTORE-IMI 2 phase 3 study.

Antimicrobial agents and chemotherapy·2026
Same author

Population Pharmacokinetic/Pharmacodynamic and Exposure-Response Modeling Analyses of Sotatercept in Healthy Participants and Patients with Pulmonary Arterial Hypertension.

Clinical pharmacology and therapeutics·2024
Same author

Discovery of sulfone containing metallo-β-lactamase inhibitors with reduced bacterial cell efflux and histamine release issues.

Bioorganic & medicinal chemistry letters·2024
Same author

Study of Functional Outcome of Intra-articular Steroid Injection and Manipulation of Shoulder Joints in Frozen Shoulder.

Cureus·2024
Same author

Population pharmacokinetic modeling of sotatercept in healthy participants and patients with pulmonary arterial hypertension.

CPT: pharmacometrics & systems pharmacology·2024

Two ADAM33 splice variants were compared in mice. The predominant variant matures correctly in lung tissue, suggesting the other variant does not exert dominant-negative effects on airway hyperresponsiveness.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Immunology

Background:

  • ADAM33 is a metalloprotease linked to airway hyperresponsiveness.
  • Two splice variants, 914 (alpha) and 906 (beta), of mouse ADAM33 exist.
  • These variants differ in exon 17, impacting protein structure.

Purpose of the Study:

  • To compare the tissue mRNA prevalence and protein maturation of mouse ADAM33 splice variants.
  • To investigate the functional implications of these variants in airway hyperresponsiveness.

Main Methods:

  • Transfection of HEK293 cells with variant cDNAs.
  • Western blotting and antibody detection of ADAM33 proteins.
  • Enzyme inhibition assays and cell surface localization studies.
  • Endoglycosidase H(f) sensitivity assays.

Related Experiment Videos

  • Quantitative RT-PCR for transcript analysis.

    • Main Results:

    • The 914 variant expressed mature (approx. 100 kD) and pro-form (approx. 120 kD) proteins, with mature form localized to the cell surface.
    • The 906 variant expressed an immature form (approx. 110 kD) retained in the trans-Golgi.
    • Transcripts containing exon 17 (914 variant) predominate in mouse lung.
    • Transcripts lacking exon 17 (906 variant) are negligible in lung but detectable in other tissues.

    Conclusions:

    • The mature, cell surface-localized form of ADAM33 is encoded by the predominant splice variant in mouse lung.
    • The non-processed 906 splice variant is unlikely to exert dominant-negative effects in mouse lung due to low prevalence and lack of maturation.