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Bioavailability and biological activity of L-ascorbic acid 2-O-alpha-glucoside
1Department of Immunochemistry, Okayama University, Japan.
Journal of Nutritional Science and Vitaminology
|January 1, 1992
Summary
A new vitamin C derivative, AA-2G, is synthesized efficiently and shows potential as a supplement. It stimulates collagen synthesis and antibody production without cytotoxicity, indicating promise for medical and nutritional applications.
Area of Science:
- Biochemistry
- Nutritional Science
- Pharmacology
Background:
- Ascorbic acid (AsA) is essential but unstable.
- Developing stable vitamin C derivatives is crucial for therapeutic and nutritional applications.
- Alpha-2-Glucosyl Ascorbic Acid (AA-2G) is a novel, stable derivative of AsA.
Purpose of the Study:
- To investigate the synthesis and biological activities of AA-2G.
- To evaluate AA-2G as a potential vitamin C supplement.
- To assess the safety and efficacy of AA-2G for medical and nutritional use.
Main Methods:
- Regioselective transglucosylation using alpha-glucosidase and CGTase for AA-2G synthesis.
- In vivo hydrolysis studies to determine AA-2G metabolism.
- In vitro assays on cultured fibroblasts and splenocytes to assess collagen synthesis and antibody production.
- Cytotoxicity assays to evaluate safety.
Main Results:
- AA-2G was efficiently synthesized and demonstrated stability.
- In vivo studies showed AA-2G is hydrolyzed by alpha-glucosidase.
- AA-2G significantly stimulated collagen synthesis in fibroblasts.
- AA-2G enhanced antibody production in splenocytes.
- AA-2G exhibited no cytotoxicity.
Conclusions:
- AA-2G is a stable and efficiently synthesized derivative of ascorbic acid (AsA).
- AA-2G acts as a viable vitamin C supplement, hydrolyzed in vivo to release AsA.
- AA-2G demonstrates beneficial biological activities, including stimulating collagen synthesis and antibody production.
- With no observed cytotoxicity, AA-2G is a promising candidate for medical and nutritional applications.