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Related Experiment Videos

A biotin-avidin based screening test for methamphetamine in urine.

C Oh1, J Kim, A Cheng

  • 1Beckman Instruments, Brea, CA 92621.

Journal of Pharmaceutical and Biomedical Analysis
|October 1, 1992
PubMed
Summary

A new biotin-avidin assay accurately screens for methamphetamine in urine. This sensitive method offers high specificity and reliability for drug testing, improving upon existing immunoassay techniques.

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Area of Science:

  • Clinical Chemistry
  • Immunology
  • Forensic Toxicology

Background:

  • Accurate and sensitive detection of methamphetamine in biological samples is crucial for clinical and forensic applications.
  • Existing screening methods may have limitations in sensitivity, specificity, or cross-reactivity.
  • Development of novel immunoassays can enhance drug detection capabilities.

Purpose of the Study:

  • To develop and validate a novel biotin-avidin based screening test for methamphetamine in urine samples.
  • To evaluate the sensitivity, specificity, and reliability of the developed assay compared to established methods.
  • To assess the cross-reactivity of the assay with structurally similar compounds like amphetamine.

Main Methods:

  • Utilized an immunoprecipitin reaction involving an antibody to methamphetamine and a biotin-avidin conjugate.

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  • Employed a biotinyl amphetamine derivative for conjugate preparation.
  • Monitored reaction rates using nephelometry on the Beckman ARRAY 360.
  • Quantified methamphetamine based on the degree of inhibition of the precipitin reaction.

    • Main Results:

    • The assay demonstrated high accuracy, correctly identifying 98.8% of GC-MS confirmed positive samples.
    • Achieved a negative prediction rate of 77.9% for GC-MS confirmed negative samples, outperforming the EMIT assay.
    • Exhibited low within-run and between-run variability (<4% RSD) near the cut-off value.
    • Showed minimal cross-reactivity (<7%) with amphetamine.

    Conclusions:

    • The developed biotin-avidin based assay is a highly sensitive and specific screening tool for methamphetamine in urine.
    • This novel assay offers improved performance compared to the EMIT assay, particularly in identifying negative samples.
    • The assay's reliability and low cross-reactivity make it a valuable method for drug testing applications.