Determination of alpha-tocopherol in semisolid gelled preparations by reversed phase HPLC

Recently there has been increasing interest in alpha-tocopherol due to the possibility of its acting as a chemoprotective agent for skin cancer since it acts to protect dermic cells from the attack of free radicals caused by ionized radiations or chemical substances. To carry out this study, semisolid preparations were made from this active substance. We demonstrate a rapid, sensitive and reproducible method of extraction and analysis using an HPLC system with ultraviolet detection to carry out routine measurements beginning with hydrophilic gels of alpha-tocopherol.