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Interaction between oleic acid-containing pH-sensitive and plain liposomes. Fluorescent spectroscopy studies.
V P Torchilin1, A N Lukyanov, A L Klibanov
1Center for Imaging and Pharmaceutical Research, Massachusetts General Hospital-East, Charlestown 02129.
FEBS Letters
|July 6, 1992
Summary
pH-sensitive liposomes interact with plain liposomes, causing fusion and oleic acid transfer. This enhances liposome permeability, aiding drug release for intracytoplasmic delivery.
Area of Science:
- Liposome interactions
- Drug delivery systems
- Biophysical chemistry
Background:
- Liposomes are crucial for drug delivery.
- pH-sensitive liposomes offer targeted delivery.
- Understanding liposome interactions is key to optimizing drug release.
Purpose of the Study:
- To investigate the interaction between pH-sensitive and plain liposomes.
- To elucidate the mechanism of oleic acid transfer between liposomes.
- To explore the implications for intracytoplasmic drug delivery.
Main Methods:
- Energy transfer method was employed.
- Studied liposome fusion dynamics.
- Analyzed changes in liposome permeability.
Main Results:
- A slow fusion process was observed between the two liposome types.
- Oleic acid transferred from pH-sensitive to plain liposomes.
- Increased permeability of both liposome types was noted, facilitating dye release.
Conclusions:
- The observed interactions and oleic acid transfer enhance liposome permeability.
- This mechanism provides insights into intracytoplasmic drug delivery using pH-sensitive liposomes.
- Oleic acid plays a role in modulating liposome behavior for drug release.