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Corneal cryo-preservation and its clinical application.
Archives D'Ophtalmologie Et Revue Generale D'Ophtalmologie
|February 1, 1975
Summary
A new, inexpensive storage medium for corneal tissue offers a promising solution to the shortage of donor corneas for transplantation. This simple method ensures clear, thin corneas suitable for keratoplasty, comparing favorably with fresh and cryopreserved tissues.
Area of Science:
- Ophthalmology
- Tissue Engineering
- Biomaterials Science
Background:
- Corneal transplantation is limited by the scarcity of donor tissue.
- Current preservation methods like cryopreservation are expensive and require specialized equipment.
- There is a need for a simple, cost-effective corneal storage solution.
Purpose of the Study:
- To develop and evaluate a simple, inexpensive tissue culture medium for short-term storage of human corneas.
- To assess the viability and suitability of corneas stored in this medium for keratoplasty.
- To compare the deturgescence rates of stored corneas with fresh and cryopreserved tissues.
Main Methods:
- Human corneas were stored for up to seven days in a novel, simple tissue culture medium.
- Stored corneas were assessed for clarity and thinness post-storage.
- Keratoplasty was performed using the stored corneas.
- Corneal deturgescence rates were measured and compared.
Main Results:
- 45 out of 55 human corneas (81.8%) remained clear and thin after seven days of storage.
- The simple storage medium proved effective in maintaining corneal integrity.
- Deturgescence rates of stored corneas were comparable to fresh and cryopreserved tissues.
Conclusions:
- A simple, inexpensive storage medium can effectively preserve corneal tissue for keratoplasty.
- This method addresses the limitations of current corneal preservation techniques.
- The developed medium offers a viable alternative for improving corneal tissue availability for transplantation.