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Cell interaction and its role in mesoderm cell migration during Xenopus gastrulation
R Winklbauer1, A Selchow, M Nagel
1Max-Planck-Institut für Entwicklungsbiologie, Abt. V, Tübingen, Federal Republic of Germany.
Summary
Mesodermal cell cohesion is crucial for Xenopus gastrulation. Aggregated cells exhibit directed migration and respond to environmental cues, unlike isolated cells, highlighting the importance of cell-cell adhesion.
Area of Science:
- Developmental biology
- Cell biology
- Xenopus laevis research
Background:
- During Xenopus gastrulation, mesoderm cells migrate collectively towards the animal pole.
- The mechanical and behavioral aspects of this collective migration are not fully understood.
Purpose of the Study:
- To investigate the role of mesodermal cell cohesion in Xenopus gastrulation.
- To determine how cell aggregate formation influences mesoderm cell migratory behavior and substrate interaction.
Main Methods:
- Observation of mesoderm cell migration in vivo.
- Comparison of migratory behavior between aggregated and isolated mesoderm cells.
- Investigation of the role of U-cadherin in mediating cell adhesion.
Main Results:
- Mesoderm cell aggregation promotes unipolar, continuous, and persistent migration.
- Only mesoderm cell aggregates, not single cells, can follow extracellular matrix guidance cues.
- Calcium-dependent cell adhesion molecule U-cadherin is implicated in mesodermal cell cohesion.
Conclusions:
- Mesodermal cell cohesion is essential for effective migration during Xenopus gastrulation.
- Aggregate formation significantly enhances the ability of mesoderm cells to navigate and respond to directional cues.
- U-cadherin plays a key role in maintaining the cohesive properties of the mesodermal cell mass.