[Identification and differentiation of the human herpes virus group using the PCR method]
R Kawaguchi1, Y Shibuya, U Ogasa
1Genetic Research Laboratory, SRL, Inc., Hachioji.
Rinsho Byori. the Japanese Journal of Clinical Pathology
|November 1, 1992
Summary
Polymerase chain reaction (PCR) offers a sensitive method for identifying and classifying human herpes viruses by amplifying virus-specific DNA. This technique detects activated viruses, aiding in diagnosing infectious diseases from latent infections.
Area of Science:
- Virology
- Molecular Biology
- Infectious Diseases
Context:
- Six types of human herpes viruses are known, classified by structure and characteristics.
- Traditional methods for virus detection include isolation and culture.
Purpose:
- To evaluate the use of Polymerase Chain Reaction (PCR) for identifying and classifying human herpes viruses.
- To compare the sensitivity of PCR with conventional virus isolation methods.
Summary:
- PCR amplifies virus-specific DNA sequences for human herpes virus identification and classification.
- The study demonstrated PCR's higher sensitivity compared to conventional methods for detecting Herpes Simplex Virus (HSV) and Cytomegalovirus (CMV).
- PCR detected only activated viruses, as indicated by negative results in normal controls, suggesting it reflects active viral replication.
Impact:
- PCR provides a more sensitive diagnostic tool for human herpes virus infections.
- The method's ability to detect activated viruses is crucial for diagnosing infectious diseases in patients with latent infections.
- This technique enhances the understanding and management of herpes virus-related illnesses.
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