Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Possible non-functional crypts in small intestine defined using mouse aggregation chimaeras.

D J Winton1, L Howard, B A Ponder

  • 1Department of Pathology, University of Cambridge, UK.

Epithelial Cell Biology
|April 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Patient-specific modelling of pulmonary arterial hypertension: wall shear stress correlates with disease severity.

Frontiers in bioengineering and biotechnology·2025
Same author

The role of shared decision-making in enhancing patient experience: Insights from a cohort of curative head and neck cancer patients.

Radiography (London, England : 1995)·2024
Same author

Systemic contact dermatitis, itching to find a cause: the penny drops.

Clinical and experimental dermatology·2021
Same author

Determinants of SARS-CoV-2 receptor gene expression in upper and lower airways.

medRxiv : the preprint server for health sciences·2020
Same author

Radiotherapy-specific interprofessional learning through simulation.

Radiography (London, England : 1995)·2020
Same author

Physician attitudes about non-medical switching to biosimilars: results from an online physician survey in the United States.

Current medical research and opinion·2019
Same journal

Stages of intestinal carcinogenesis and their control by host NK cells. Implications to prevention and therapy.

Epithelial cell biology·1995
Same journal

Gamma delta TCR-bearing intraepithelial lymphocytes regulate class II major histocompatibility complex molecule expression on the mouse small intestinal epithelium.

Epithelial cell biology·1995
Same journal

Effects of the excretory/secretory products of six nematode species, parasites of the digestive tract, on the proliferation of HT29-D4 and HGT-1 cell lines.

Epithelial cell biology·1995
Same journal

Effects of keratinocyte-secreted soluble factors on spreading, number of dendrites and cell-cell contacts of human epidermal melanocytes and dermal fibroblasts: a quantitative analysis.

Epithelial cell biology·1995
Same journal

Effects of the medium HCO3-/CO2 buffer system on differentiation and intermediary metabolism properties of rabbit proximal tubule cells in primary culture.

Epithelial cell biology·1995
Same journal

Transitional differentiation patterns of principal and intercalated cells during renal collecting duct development.

Epithelial cell biology·1995
See all related articles

A small population of intestinal crypts were found to not export cells to villi in mouse models. This discovery offers new insights into small intestine cell dynamics and tissue regeneration.

Area of Science:

  • Gastroenterology
  • Developmental Biology
  • Cell Biology

Background:

  • The small intestine's epithelial lining is continuously renewed.
  • Intestinal crypts are the stem cell niches responsible for this renewal.
  • Cellular export from crypts to villi is a key process in maintaining intestinal homeostasis.

Purpose of the Study:

  • To investigate the cellular dynamics of intestinal crypts.
  • To identify any crypt populations with atypical cell export behavior.
  • To understand the implications for small intestine tissue regeneration.

Main Methods:

  • Utilized C57BL/6J<-->SWR mouse aggregation chimeras.
  • Employed whole-mount small intestine preparations.
  • Stained tissues with Dolichos biflorus agglutinin peroxidase conjugate (DBA-Px) for epithelial cell identification.

Related Experiment Videos

Main Results:

  • Identified a minor population (13%) of isolated C57BL/6J crypts that did not export cells to villi.
  • Observed these crypts surrounded by non-staining SWR epithelium.
  • Noted that these crypts had surrounding C57BL/6J epithelium on the intervillus gut floor.

Conclusions:

  • A subset of intestinal crypts may function independently of villus cell export.
  • This finding suggests heterogeneity in crypt stem cell behavior.
  • Further research is needed to elucidate the mechanisms and implications of non-exporting crypts in small intestine regeneration.