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Related Experiment Videos

Capillary hydraulic conductivity is elevated by cGMP-dependent vasodilators.

D J Meyer1, V H Huxley

  • 1Department of Physiology, University of Missouri School of Medicine, Columbia 65212.

Circulation Research
|February 11, 1992
PubMed
Summary

Agents that increase cyclic guanosine monophosphate (cGMP) levels, such as atrial natriuretic peptide (ANP) and sodium nitroprusside (SNP), significantly elevate capillary hydraulic conductivity (Lp). This study confirms cGMP

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Area of Science:

  • Physiology
  • Microcirculation Research
  • Vascular Biology

Background:

  • Microvascular functions are influenced by agents affecting cyclic nucleotide levels.
  • Capillary exchange capacity, measured by hydraulic conductivity (Lp), is a key microvascular parameter.
  • Elevated cellular cyclic guanosine monophosphate (cGMP) levels are hypothesized to increase Lp.

Purpose of the Study:

  • To investigate the effect of cGMP-elevating agents on frog mesenteric capillary hydraulic conductivity (Lp).
  • To determine if atrial natriuretic peptide (ANP) and sodium nitroprusside (SNP) increase Lp.
  • To assess the role of cGMP in mediating changes in capillary permeability.

Main Methods:

  • Measurement of frog mesenteric capillary Lp under luminal exposure to various agents.

Related Experiment Videos

  • Administration of ANP, truncated atriopeptins (ANP-I, ANP-III), SNP, and a cGMP phosphodiesterase inhibitor (M&B 22948).
  • Evaluation of agent effects alone and in combination with methylene blue dye or ANP/SNP.

    • Main Results:

    • ANP (10-100 nM) and SNP (1 microM) significantly increased Lp (2.1-2.6 fold).
    • ANP-III increased Lp (1.7 fold), while ANP-I had no significant effect.
    • The cGMP phosphodiesterase inhibitor M&B 22948 also elevated Lp (2.8 fold).
    • SNP-induced Lp increase was inhibited by methylene blue, but ANP's effect was not.
    • Methylene blue alone did not affect Lp.

    Conclusions:

    • Agents that elevate cellular cGMP levels, including ANP and SNP, increase capillary hydraulic conductivity (Lp).
    • These findings support the hypothesis that cGMP mediates an increase in capillary exchange capacity.
    • The results provide strong evidence for the role of cGMP in regulating microvascular permeability.