Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hormonal regulation of major histocompatibility complex class I genes in rat thyroid FRTL-5 cells:

M Saji1, J Moriarty, T Ban

  • 1Laboratory of Biochemistry and Metabolism, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892.

Proceedings of the National Academy of Sciences of the United States of America
|March 1, 1992
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Improving recruitment to occupational health professions through highlighting intrinsic rewards.

Occupational medicine (Oxford, England)·2024
Same author

Large fluctuations in locational marginal prices.

Philosophical transactions. Series A, Mathematical, physical, and engineering sciences·2021
Same author

Detecting changes in real-time data: a user's guide to optimal detection.

Philosophical transactions. Series A, Mathematical, physical, and engineering sciences·2017
Same author

A synthetic review of notoedres species mites and mange.

Parasitology·2016
Same author

Fatal Pancreatic Necrosis after Pregnancy-Associated Thrombotic Thrombocytopenic Purpura.

Hematology (Amsterdam, Netherlands)·2016
Same author

Role of cardiopulmonary exercise testing as a risk-assessment method in patients undergoing intra-abdominal surgery: a systematic review.

British journal of anaesthesia·2016

Thyroid-stimulating hormone (TSH) reduces major histocompatibility complex (MHC) class I expression in thyrocytes. This decrease in MHC class I is linked to reduced TSH receptor expression and is mediated by the cAMP pathway.

Area of Science:

  • Endocrinology
  • Immunology
  • Molecular Biology

Background:

  • Thyrocytes express major histocompatibility complex (MHC) class I but not class II antigens.
  • Thyroid-stimulating hormone (TSH) regulates thyroid-specific gene expression and TSH receptor levels.
  • TSH-induced reduction in TSH receptor expression is mediated by cyclic adenosine monophosphate (cAMP).

Purpose of the Study:

  • To investigate the effect of TSH on MHC class I expression in thyrocytes.
  • To determine if TSH-mediated changes in MHC class I expression correlate with TSH receptor expression.
  • To elucidate the molecular mechanisms underlying TSH's regulation of MHC class I gene transcription.

Main Methods:

  • Utilized the rat thyrocyte cell line FRTL-5.
  • Treated FRTL-5 cells with physiological concentrations of TSH.

Related Experiment Videos

  • Assessed MHC class I expression via cell surface antigen levels, steady-state RNA, and gene transcription.
  • Mapped the TSH-responsive region in the MHC class I gene promoter.

    • Main Results:

    • TSH treatment decreased both MHC class I and TSH receptor expression in FRTL-5 thyrocytes.
    • Reduced MHC class I expression was observed at the cell surface, RNA, and transcriptional levels.
    • TSH-mediated reduction in MHC class I gene transcription was localized to a region within 135 base pairs of the promoter.

    Conclusions:

    • TSH significantly downregulates MHC class I expression in thyrocytes.
    • The downregulation of MHC class I by TSH is associated with decreased TSH receptor expression.
    • TSH influences MHC class I gene transcription through a proximal promoter region, suggesting a complex regulatory interplay within thyroid cells.